Ceramide assay kit

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August undefined, 2024

WebThis ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the targets. This ELISA kit for research use only, not for therapeutic or diagnostic … WebNov 2, 2024 · To evaluate the in vitro BBB permeability of plant ceramides, a Rat BBB Culture Model Kit (PharmaCo-Cell Co. Ltd., Nagasaki, Japan), ... Schematic representation of the in vivo assay. Synthetic plant-type ceramides were injected intravenously into mice. After 30 min, the brain was removed and analyzed by LC-MS/MS. (B) Chemical structure …

Ceramide Mediates Insulin Resistance by Tumor Necrosis Factor-α …

WebExoELISA Complete Kit (CD63 Detection) A sensitive ELISA assay for exosome quantitation based on the presence of CD63, a general exosome marker. Compatible with most biofluids. Sensitive—detect as little as 500 µg protein equivalent Flexible—compatible with all major exosome isolation methods (g. WebCell Biolabs’ Sphingomyelin Assay Kit is a simple fluorometric assay that measures the amount of sphingomyelin present in plasma or serum, tissue homogenates, or cell … holistic tagalog

GW4869 = 90 NMR 6823-69-4 - Sigma-Aldrich

WebThis kit recognizes human BDNF protein and is cross-reactive to mouse and rat BDNF protein. ASSAY SPECIFICITY This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit. CROSS REACTIVITY WebNov 1, 2001 · After the addition of 1 ml of 1 mol/l NaCl and 1 ml of CHCl 3 and further mixing, phases were separated by centrifugation at 5,000g for 2 min. Lipid extracts in the lower phase were assayed for ceramide content using a radiometric assay kit according to the manufacturer’s instructions, as DAG kinase can phosphorylate both DAG and … WebMar 21, 2003 · After the subsequent addition of 0.25 ml of 1 m NaCl and 0.25 ml of CHCl 3, phases were separated by centrifugation at 3000 rpm (2000 × g) for 5 min. Ceramide content in the extract was determined using a radiometric diacylglycerol assay kit (Amersham Biosciences) according to the manufacturer's instructions. human differential

Sphingosine 1-Phosphate ELISA Kit - Echelon Biosciences

Mouse Ceramide ELISA kit, AMS.E03C2522 AMSBIO

WebFeb 21, 2024 · The CerS Assay Kit offers a number of advantages including: a short assay time, a requirement of only small amounts of biological material, and elimination of the … WebTo achieve ER labeling, this kit employs a primary antibody directed against an ER-associated protein, protein disulfide isomerase (PDI), and an Alexa Fluor 488 dye–labeled secondary antibody. The Alexa Fluor 488 dye exhibits bright green fluorescence that is compatible with filters and instrument settings appropriate for fluorescein. holistic tantraWebCeramide is a sphingolipid which regulates a variety of signaling pathways in eukaryotic cells. Exogenous ceramide has been shown to induce cellular apoptosis. human diet its origin and evolution

"WebOct 22, 2024 · Ceramide labeling in patient-derived fibroblasts showed an altered distribution of ceramide indicated by the increased puncta compared to control … " - Ceramide assay kit

Ceramide assay kit

A fluorescence-based high-performance liquid chromatographic assay …

WebThe Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate. Assay sensitivity: 25 pmol Sample … WebGW4869 is a commonly used pharmacological agent, which inhibits exosome generation. It blocks ceramide-mediated inward budding of multivesicular bodies (MVBs) and the release of mature exosomes from MVBs. [ 1] GW4869 exhibits cytotoxicity to phosphatidylserine-expressing myeloma cells. [ 2] It inhibits the secretion of IFN (interferon)-α by ...

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WebApr 12, 2024 · Plasma insulin was determined using Ultra-Sensitive Mouse Insulin ELISA Kit (Crystal Chem, USA) and plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined using Infinity reagents (ThermoFisher Scientific, USA). WebThe CerS Assay Kit provides a simple and high-throughput assay for measuring CerS activity in biological samples. This as-say uses NBD sphinganine and a 18:0 (CerS1/4), …

WebThis Human Phosphatidylcholine:ceramide cholinephosphotransferase 1 (SGMS1) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. WebSep 15, 2003 · This new ceramide kinase assay is more specific than the commonly used diacylglycerol kinase method because the ubiquitous lipid diacylglycerol, the preferred substrate for diacyglycerol kinase which is usually present at higher concentrations than ceramide and can interfere with ceramide phosphorylation, is completely inactive with …

WebThe CerS Assay Kit provides an easy to use assay for measuring CerS activity in biological samples. This Kit offers the advantage of a short assay time requiring only small amounts of biological material while eliminating TLC as a separation technique with its attendant degradation of NBD sphinganine. Packaging Kit contains: WebMay 17, 2013 · Ultraviolet (UV) radiation and reactive oxygen species (ROS) impair the physiological functions of retinal pigment epithelium (RPE) cells by inducing cell apoptosis, which is the main cause of age-related macular degeneration (AMD). The mechanism by which UV/ROS induces RPE cell death is not fully addressed. Here, we observed the …

WebApr 13, 2024 · The literature data indicate that the cellular levels of sphingosine-1-phosphate (S1P) and ceramides (Cer) play a significant role in the pathogenesis of CRC, and the S1P/Cer ratio (described as sphingolipid rheostat) decides whether the cells will be directed towards proliferation or cellular senescence and apoptosis [ 6, 7 ].

WebCell Navigator® TMR Ceramide Golgi Staining Kit provides an optimized assay method for examining the morphology of the Golgi apparatus with a fluorescence microscope. In … holistic tails cbdWebUDP Glucose Ceramide Glucosyltransferase (UGCG) BioAssay™ ELISA Kit (Mouse, Rat and Human) Detection Target: UDP Glucose Ceramide Glucosyltransferase. Quantity: … 249 reviews. Compare ELISA Kit Search from leading suppliers on Biocompare. … holistic talk therapyWebPolyclonal Antibody to Ceramide Kinase (CERK) WB; IHC; ICC; IP. MAJ459Hu21: Monoclonal Antibody to Ceramide Kinase (CERK) WB; IHC; ICC; IP. SEJ459Hu: ELISA … holistic tarotWebThis study investigated the protective effects of glucocerebroside-containing buttermilk concentrated powder (GCBM) on oxidative stress and photoaging in ultraviolet B (UVB)-irradiated hairless mice. We measured antioxidant enzyme activities, collagen synthesis-related pathways, and moisturizing-related factors in the dorsal skin of mice. We … human differences meaningWebJan 7, 2024 · Ceramide measurement Ceramide was measured as described previously 36. After the treatment, cells were washed with PBS and lysed using ceramide assay lysis buffer (50 mM Tris pH-7.4... human dichotomyWebThe Qubit Protein Assay Kits are designed to make protein quantification easy and accurate. Addressing overlapping concentration ranges, the two kits together are accurate for initial sample concentrations from 12.5 μg/mL to 20 mg/mL and exhibit low protein-to-protein variation. holistic tappingWebCytotoxicity Assay. The cytotoxicity of PEG-ceramide nanomicelles against N2a cells was measured using a CCK-8 kit. Briefly, N2a cells (dissociated into single cells) were seeded in 96-well plates at a density of 3×10 3 cells per well and incubated overnight. The cells were then incubated for 24 h with varying concentrations of PEG-ceramide ... human digestion year 4