Ctll 2 assay

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August undefined, 2024

WebFeb 13, 2013 · A toxicogenomics approach was applied to assess the usefulness of the mouse cytotoxic T cell line CTLL-2 for in vitro immunotoxicity testing. CTLL-2 cells were exposed for 6 h to two model immunotoxic compounds: (1) the mycotoxin deoxynivalenol (DON, 1 and 2 μM), a ribotoxic stress inducer, and (2) the organotin compound tributyltin … WebED 50 =2.5 - 15 ng/ml, corresponding to a specific activity of 0.6 - 4 x 10 5 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation. Application Bioassay. Application Notes This IL-15 protein is biologically active and can be used for in vitro assays. Product Citations. Gupta SS, et al. 2024. Cell Rep. 29:1862.

ECACC General Cell Collection: 93042610 CTLL-2 - Culture …

http://immunservice.com/wp-content/uploads/User-Manual-WST-1-CTLL-2-WST1.CTLL150813-EN-V4.pdf WebInstructions for Use of Product(s) J1651, J1655, J1657, J1656. Literature # TM492. The T Cell Activation Bioassay (IL-2), is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used for the discovery and development of novel biologic and cell therapies aimed at inducing, strengthening and/or engineering T cell … fixed blade knife with finger guard

Recombinant Mouse IL-15 carrier-free - BioLegend

WebPromega ctll 2 proliferation assay Ctll 2 Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebNov 22, 2007 · It relies on the IL-2- or IL-4-dependence of a cytotoxic cell line (CTLL-2) for in vitro proliferation, and the measurement. of the growth of the these cells is usually … WebCTLL, CTLL2, CTLL(2), CT, CTL2, CTL-2, TLL 2 Mouse C57b1/6 T cell CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media. Mouse Blood Gillis S, Smith KA. 1977 Long term culture of tumour-specific cytotoxic T cells. Nature 268:154-6 PMID: 145543 … can man travel at the speed of light

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CTLL-2 93042610 Sigma-Aldrich

WebDownload scientific diagram CTLL-2 cells proliferation assay of purified superagonist to evaluate its bioactivity. The EC 50 was calculated by the four-parameter fit logistic equation to be 30 ... WebNov 29, 2013 · 2.2. Assay methods and study design. ... CTLL-2 or the HT-2 cell-line but employed different readouts for assessing the proliferation (Gillis et al., 1978, Gieni et al., 1995). In one laboratory, however, the human T cell … fixed blade knife shapesWebIL-2 : CTLL-2 (ATCC TIB-214) 2: 24: 10: ≤175pg/mL: Proliferation: IL-3 : TF-1 (ATCC CRL-2003) 2: 48: 10: ≤0.6-0.15ng/mL: Proliferation: IL-4 : TF-1 (ATCC CRL-2003) 2: 48: 10: ... Cytotoxicity Assay: VEGF 121: HUVEC: 2: 72: 1000: ≤8-10ng/mL: Proliferation: Mouse Cytokine Bioassay Quick Guide Cytokine Indicator Cells Cell Density (x10 5) fixed blade knife sheaths leather

"WebCTLL-2 cells were seeded in a 96-well plate and treated with varying concentrations of Human Recombinant IL-2. Cell proliferation was measured using a colorimetric assay. In the graph below, the specific activity was determined … " - Ctll 2 assay

Ctll 2 assay

General Cell Based Assays Thermo Fisher Scientific

WebPreparation of CTLL-2 Cell Assay Medium Reagent Amount . RPMI 1640 medium (3.1) 450 mL . Heat-inactivated FBS (3.2) 50 mL . Label as CTLL-2 Cell Assay Medium with the … WebCtll 2 Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, …

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Web本发明涉及包含人白介素‑15(il‑15)的突变体的疫苗组合物。本发明还涉及所述人il‑15突变型多肽在制备药物中的用途，所述药物用于与il‑15的过表达相关的疾病的主动免疫疗法，所述与il‑15的过表达相关的疾病包括某些自身免疫疾病和血液学瘤形成。向有此需要的个体施用治疗有效量的包含本 ... WebApr 1, 2024 · CTLL-2 cells proliferation assay of purified superagonist to evaluate its bioactivity. The EC 50 was calculated by the four-parameter fit logistic equation to be 30 pM. 3.4. Prolonged half-life of IL-15·sIL-15Rα/Fc. The half-life of circulating IL-15 in blood is critical for its in vivo bioactivities.

WebCellTiter-Glo 2.0 Assay will maintain >90% activity upon storage at 4°C for 4 weeks or >85% activity upon storage at room temperature for 7 days. The CellTiter-Glo 2.0 Assay … Web6.4 Label the complete medium as CTLL-2 Cell Culture Medium with the lot number, date prepared, initials, and expiry date based on the component with the shortest expiration …

WebOct 31, 2000 · In this study, alamarBlue was used to measure the proliferation of the IL-2-dependent cytotoxic T cell line, CTLL-2. The colorimetric change of alamarBlue at 570 nm compared to the reference wavelength, 600 nm, was proportional to the number of viable cells. The sensitivity of the IL-2 assay using alamarBlue was comparable to that of the … WebMay 18, 2024 · CTLL-2 cells (AATC TIB-214), a murine IL-2-dependent T cell line, were obtained from the vendor and maintained in complete RPMI supplemented with 1000 IU/mL recombinant mouse IL-2. ... Furthermore, the in vitro suppression assay graph in Figure 6—figure supplement 1 is insufficient without a "No Treg" control to evaluate the degree …

WebImmunservice’s WST-1 CTLL-2 cell proliferation Kit provides an easy, fast and accurate method to determine cell proliferation, cell viability and cytotoxicity. The Kit was specifically optimized for applications with CTLL-2 cells. The cytotoxic murine T-cell line CTLL-2 is well-suited and frequently used to assess the biological activity of the

fixed blade knife with cross draw sheathWeb(2 X lo6 cells/ml) were cultured in the presence of either rIL-2 or the mutant proteins at 37 "C for 88 h. After incubation, the supernatant was collected and assayed for IFN activity. CTLL-2 Proliferation Assay-The proliferation assay was deter- mined by the incorporation of 3H-labeled thymidine into CTLL-2 cells as described previously (6). fixed blade knife wood handleWebSupport protocols are provided for the quantitation of CTLL-2, CT4.S, or CT.h4S proliferation using a standard [3H]thymidine incorporation method or by using the 3-(4,5 … can manticore be tamedWebJun 2, 2012 · Another well-known bioassay utilizes a CTLL-2 cell line as a target for measuring proliferative activity of IL-2 (Gillis et al., 1978) ... The selection of the … can man travel through the van allen beltWebThe present invention relates to cancer immunotherapy. In particular, provided herein are fusion proteins for targeting tumor associated macrophages with immunostimulatory agents. can manufactured homes go vaWebApr 11, 2024 · gamma chain γc/IL2 Rγ, which is shared with the receptors for IL4, 7, 9, 15, and 21, does not independently interact with IL2. Upon ligand binding, signal transduction is performed by both IL2 Rβ and γc. IL2 is best known for its … fixed blade knife with knuckle guardWebJun 9, 2024 · A CTLL-2 cell proliferation assay was used to evaluate the biological activity of the rhIL-15 and IL-15/SuIL-15Rα-IgG4 Fc complexes. The EC 50 values were calculated using the four-parameter nonlinear logistic regression model. All data points are the means ± standard deviation of triplicate OD values. The results are representative of at ... fixed blade knife with finger ring