Fluo-4 am thermo fisher scientific
WebApr 12, 2024 · 细胞活力&增殖&凋亡:Calcein-AM细胞活力测定,ViaFluor细胞增殖,JC-1,TMRM & TMRE,MitoView等检测线粒体膜电位 ... 钙离子&其他离子指示剂:Fluo-3,Fluo-4,Fura-2,Bis-fura-2,Furaptra,Indo-1 ... 下图为Biotium公司授权艾美捷科技有限公司(AmyJet Scientific Inc.)作为Biotium在中国 ... WebThis green fluorescent calcium indicator, fluo-4 AM, is an improved version of the calcium indicator, fluo-3, AM. The fluo-4, AM loads faster, is brighter at equivalent concentrations and is well-excited by the 488 nm line of the argon-ion laser. T ... Thermo Fisher Scientific. About Us ; Careers ;
Fluo-4 am thermo fisher scientific
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WebThe Fluo-4 NW Calcium Assay provides the same advantages as Fluo-4 AM but with the added convenience of an improved formulation that includes PowerLoad Concentrate, an improved dye loading reagent, and eliminates the need for washing steps after media removal. Figure 1. CHO M1 response to carbachol. Table 1. WebDescription. Fluo-4 AM is a cell-permeable Ca 2+ indicator [1] . In Vitro. Fluo-4 AM is a fluorescent dye (λ ex =494 nm, λ em =516 nm). …
WebFeb 27, 2024 · For simultaneous monitoring of the calcium dynamics and the cell membrane and microstructure, the cells were dual-labelled with cell-permeant calcium indicator Fluo-4 AM (Thermo Fisher Scientific ... WebThe measurement of intracellular calcium ions was conducted as previously described , with some modifications: 4.0 × 10 6 Jurkat cells suspended in Dulbecco’s phosphate-buffered saline without magnesium chloride and calcium chloride (D-PBS (-), FUJIFILM Wako Pure Chemical) were incubated with 4.5 μM Fluo-4 AM (Thermo Fisher Scientific) for ...
WebFluo-4 Calcium Imaging Kit Catalog number: F10489 664.00 / 1 kit Add to cart Fluo-4, AM, cell permeant Catalog number: F14217 312.00 / 500 µL Add to cart Documents & Downloads Certificates Lot #Certificate TypeDateCatalog Number (s) 2351969 Certificates of AnalysisJun 15, 2024F14222 2061414 Certificates of AnalysisJan 29, 2024F14222 WebFluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Web1.2 主要试剂 DMEM/F12基础培养基购自上海源培生物科技有限公司;胎牛血清、0.25%胰蛋白酶和青链霉素混合液购自Hyclone;氰戊菊酯、N-乙酰基-L-半胱氨酸(NAC,ROS清除剂)购自Sigma;人绒毛膜促性腺激素购自上海阿拉丁生物科技有限公司;BAPTA-AM(胞内Ca2+离子螯合剂)购 ...
WebSections were then mounted on slides and covered with anti-fade mounting medium (ProLong; Thermo Fisher Scientific). Table 1. Antibodies Used for Flow Cytometry and Immunofluorescence Staining. Catalog no. Name Company; Antibodies used for flow cytometry ... potential cells were loaded with Fluo-4 AM and spontaneous calcium … flint acorn patio benchWebFluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. greater jobs teachingWebL’indicateur de calcium fluorescent vert , fluo-4 AM, est une version améliorée de l’indicateur de calcium, fluo-3 AM. Le fluo-4 AM charge plus rapidement, est plus lumineux à des concentrations équivalentes et est bien excité par la ligne de 488 nm du laser argon-ion ... Search Thermo Fisher Scientific. Rechercher . Rechercher. Statut ... greater jobs youth justiceWebFluo-4, AM, cell permeant Popular Applications & Techniques Shop All Products Services Support Contact Us Quick Order Sign In Search All Error: We're sorry but this product is not available at this time. greater jobs wigan council loginWebJan 17, 2024 · The lower chamber of the transwell was filled with 600 μL of culture medium containing EGF (5 μg mL −1, Thermo Fisher Scientific). After 24 h incubation, inserts were removed and fixed by 4% PFA for 15 min at RT and stained by Hoechst33342. Non-invading cells that remained on the top of the transwell were scraped by a cotton swab. greater johnstown aysoWebCalcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. flint aero tip tanksWebFigure 1. Time-lapse imaging and quantitation of cardiomyocyte pulse rate.(A) Time-lapse images (obtained from a video of contracting cardiomyocytes stained using the fluorescent fluo-4 calcium indicator provided in the Invitrogen Fluo-4 NW Calcium Assay Kit and recorded using the Invitrogen EVOS FL Auto 2 Imaging System show the flux of calcium … flint ag and turf leesburg ga