WebFeb 21, 2024 · Genomic DNA was extracted using a commercial kit (QIAGEN Gentra Puregene Yeast/Bact Kit, Germany) in accordance with the manufacturer’s instructions. The library for single-molecule real-time (SMRT) sequencing was constructed (insert size 10 kb) using the SMRT Bell Template kit version 1.0; a next-generation sequencing library … WebJun 26, 2024 · Chromosomal DNA was isolated using the Gentra Puregene Yeast/Bact. kit (Qiagen, Hilden, Germany) with some modifications. After washing the cell pellet, cells were resuspended in cell lysis solution and transferred into screw cap tubes containing 200 μL of zirconia/silica beads (0.5 mm, Daintree Scientific, Tasmania, Australia).
Gentra Puregene Handbook - GGBC
WebGentra Puregene Yeast/Bact kits (QIAGEN) were used to purify DNA from cells in late log-phase. Genomic DNA libraries were prepared using the Nextera DNA Sample Preparation Kit (Illumina) with some modifications to the standard protocol to increase throughput, including custom barcodes, smaller reaction sizes, and additional size-selection steps ... WebGentra Puregene Blood Kits* — for purification of archive-quality DNA from whole blood and bone marrow Gentra Puregene Blood Kit (3 ml) For 3 ml blood: RBC Lysis … blockbench walking animation
Gentra Puregene Handbook - Emmes
WebHi, I intend to extract DNA from gram positive bacteria (bacillus bacillus to be specific) by using this Qiagen kit (Gentra Puregene Yeast/Bact. Kit). We intend to sequence the DNA. It comes packaged with a "Lytic Enzyme Solution" (which contains Lyticase) for the initial cell lysis step of the protocol. Web1. Deep in Ink Tattoos. “First time coming to this tattoo parlor. The place was super clean and all the tattoo needles he used were sealed and packaged. He opened each one in … WebProduct Details. Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references. To improve availability and address demand more efficiently we implemented ... blockbench下载