Phosphinothricin acetyltransferase gene
WebDownload scientific diagram Schematic structure of the T-DNA region of the binary vector pCAMBIA3301. LB, left border; RB, right border; bar, phosphinothricin acetyltransferase gene; gus-int, β ... WebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase ( PAT) and bialaphos resistance ( bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application.
Phosphinothricin acetyltransferase gene
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WebJan 1, 1995 · Phosphinothricin is an inhibitor of glutamine synthetase, which plays a central role in the assimilation of ammonia and in the regulation of the nitrogen metabolism in plants. 9, 10 It is the only enzyme that detoxifies ammonia produced during nitrate reduction, photorespiration, and amino acid degradation in plant cells. WebGene: pat. Gene Source: Streptomyces viridochromogenes. Product: phosphinothricin N-acetyltransferase (PAT) enzyme. Function: eliminates herbicidal activity of glufosinate …
WebMar 9, 2024 · Plasmid pGL2, bearing the hygromycin phosphotransferase gene ( hph) coding sequence under control of the CaMV35S promoter, is used for hygromycin selection [ 12 ]. Plasmid DNA is isolated using a modified cetyltriethylammoniumbromide (CTAB) extraction method [ 13 ]. Plasmid DNA is dissolved in water and stored at −20 °C. WebThis gene encodes neomycin phosphotransferase, which confers resistance to antibiotics such as kanamycin, gentamicin A, neomycin, and others when expressed in plants. The hpt gene, also isolated from E. coli, codes for hygromycin transferase and confers resistance …
WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. WebThe gene encodes phosphinothricin acetyltransferase, which N-acetylates phosphinothricin using acetyl-CoA. Figure 6.1 . Overview of biosynthetic pathways of a number of …
WebNov 27, 2024 · Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also …
WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from … share audible books with friendsWebSKU: GENE-BT-CP4 Category: Foody Safety Assays Tag: plantExtract. Related products. Ovine DNA Real Time PCR Detection Kit. Read more. cp4Epsps Gene Rapid Test Kit. ... phosphinothricin-N-acetyltransferase Test pAT/cp4 Epsps combo Test. Scroll to top. This site uses cookies. By continuing to browse the site, you are agreeing to our use of ... share a tweet on twitterWebFeb 16, 2016 · Wohlleben, W. et al. Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene 70 , 25–37 (1988). pool heater keeps shutting offWebThe gene encodes phosphinothricin acetyltransferase, which N -acetylates phosphinothricin using acetyl-CoA. Sign in to download full-size image Figure 6.1. Overview of biosynthetic pathways of a number of phosphonate and phosphinate natural products. The first two steps of phosphonate biosynthesis (boxed) are conserved for most known … share audible accountWebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … share auctionWebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) tiafenacil in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said … share a tweet on facebookWebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) … pool heater installation youngstown oh