Phosphinothricin acetyltransferase
WebMay 26, 2024 · Phosphinothricin acetyltransferase (PAT), the target protein (Kim et al. 2010) investigated in this study, is a typical herbicide-resistant protein coded by a bar gene isolated from Streptomyces hygroscopicus …
Phosphinothricin acetyltransferase
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WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … WebApr 11, 2024 · phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also contains the gene that encodes for the phosphomannose isomerase protein, which is used as a selectable marker. The petition states that DP23211 corn is unlikely to pose a plant pest risk and, therefore, should
WebPhosphinothricin acetyltransferase is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase.[1][2] This enzyme catalyses the following … WebApr 1, 2005 · DP23211 maize expresses an insect-specific dsRNA, an insect-active non-Bt protein, the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate …
WebOct 1, 1996 · The bar and pat genes, isolated from different Streptomyces species, both encode a phosphinothricin acetyltransferase (PAT) and are widely applied in plant … WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) tiafenacil in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said …
WebFeb 14, 2024 · Phosphinothricin acetyltransferase (PAT) and the genetic material necessary for its production in all plants are exempt from the requirement of a tolerance when used as plant-pesticide inert ingredients in all plant raw agricultural commodities.
Web2.2 Preparation of the Phosphinothricin Acetyltransferase Electrochemical Immunosensor Prior to modification, the GCE was polished with 1 μm, 0.3 µm, and 0.05 µm aluminum powder sequentially on polishing paper, with sonication in double-distilled water between each polishing step. reached budgetWebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... how to start a hypothesis statementWebIt codes for phosphinothricin acetyl transferase (PAT) proteins of 183 amino acids and shows 85% DNA sequence homology with another marker gene pat isolated from Streptomyces viridochromogenes. how to start a icloud accountWebPPT N-acetyltransferase. acetyl-CoA + phosphinothricin <=> CoA + H (+) + N-acetylphosphinothricin. The substrate phosphinothricin is used as a nonselective herbicide and is a potent inhibitor of EC 6.3.1.2, a key enzyme of nitrogen metabolism in plants. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in ... reached by a stairwayWebApr 15, 2024 · Here we demonstrate that purified PpArsN1 exhibits phosphinothricin acetyltransferase activity (Table 1). The glutamine synthetase inhibitor L-MSO is a poorer substrate compared with AST (Fig. 1d ). reached by allie condieWebGene: pat Gene Source: Streptomyces viridochromogenes Product: phosphinothricin N-acetyltransferase (PAT) enzyme Function: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation GM Trait: Glufosinate herbicide tolerance Events with gene pat how to start a incorporationWebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. reached by plane crossword