Phospho mtor antibody

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August undefined, 2024

WebMTOR (2475) Expression: P42345 MTOR_HUMAN: Expressed in numerous tissues, with highest levels in testis. Description: an atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. WebSep 5, 2024 · p-mTOR Antibody (59.Ser 2448) is an IgG1 κ mouse monoclonal p-mTOR antibody that detects Ser 2448 phosphorylated mTOR of mouse, rat and human origin by …

Anti-phospho-mTOR (Thr2446) Antibody - Sigma-Aldrich

WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … WebPhospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389. This antibody also detects p85 S6 kinase when phosphorylated at the analogous site … small dining table with chairs

Western Blot Detection of Phosphorylation Events - Bio-Rad

WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for … WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. WebMTOR (2475) Expression: P42345 MTOR_HUMAN: Expressed in numerous tissues, with highest levels in testis. Description: an atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. so near so far musings for miles

Phospho-mTOR (Ser2481) Polyclonal Antibody - Thermo Fisher …

p-mTOR Antibody (59.Ser 2448) SCBT - Santa Cruz …

WebAnti-mTOR (phospho T2446) antibody See all mTOR primary antibodies Description Rabbit polyclonal to mTOR (phospho T2446) Host species Rabbit Tested applications Suitable for: WB more details Species reactivity Reacts with: Mouse Immunogen Synthetic peptide corresponding to Human mTOR (phospho T2446). Database link: P42345 General notes WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit Synonym (s): FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1 eCl@ss: 32160702 NACRES: NA.41 Pricing and availability is not currently available. Properties biological source rabbit Quality Level 100 antibody form affinity purified immunoglobulin sonec flywayWebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies Cells (fibroblast, MRC-5 human cell lines) were starved form 4 hours (0,5% FBS) and induced with TGF-b. eIF4E siRNA was transfected with lipofectamine RNAmax for 24 hours. so near but so far away

"WebAnti-mTOR (phospho S2448) antibody [EPR426 (2)] See all mTOR primary antibodies Description Rabbit monoclonal [EPR426 (2)] to mTOR (phospho S2448) Host species Rabbit Tested applications Suitable for: WB, IHC-P, … " - Phospho mtor antibody

Phospho mtor antibody

Recombinant Anti-mTOR (phospho S2448) antibody [EPR426(2)]

WebAn antibody to the phosphorylated S6 ribosomal protein was used to stain the tumors, all high-grade sarcomas. Pretreatment biopsy or resection material was tested: the original tumor (n=6) or tumor recurrence/metastasis (n=14); either of these may have been after treatment with other agents. Webtracts were immunoprecipitated with rabbit -mTOR antibody or-AU1 antibody. Immune complexes were collected on protein A-Sepha-rose (Sigma) or -mouse IgG-agarose (Sigma), washed three times with ... tion—Previous reports demonstrated that mTOR was phospho-rylated in vitro by AKT at a serine residue (Ser-2448) located in studies, we ...

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WebPhosphoPlus® mTOR (Ser2448) Antibody Duet PhosphoPlus ® mTOR (Ser2448) Antibody Duet #39182 Reviews () Citations (2) Flow cytometric analysis of A549 cells using mTOR (7C10) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed line). WebThe Phospho-mTOR (Ser2481) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2481) Antibody has been validated for the following applications: Western Blot.

WebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide … Web4% PFA plus methanol. Methanol only. This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and …

Web2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389), Phospho-S6K (Thr-421/424), total S6K1, phospho-4E-BP1 (Thr-37/46), and total 4E-BP were diluted … WebDownload Zoom Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in Dot Blot, Western Blotting. Anti-phospho-mTOR (Thr2164) Antibody MSDS …

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly …

WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT/ GSK3 beta / mTor and total-AKT/ GSK3 beta / mTor. For each target, a capture antibody has been coated onto microwells. small dining table with bench and chairsWebWestern blot analysis of NIH/3T3, using Phospho-mTOR-S2448 Rabbit mAb (AP1413) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in … soneath pond mdWebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ... sonecha and amlaniWebProduct name Anti-mTOR (phospho S2481) antibody [EPR427 (N)] See all mTOR primary antibodies Description Rabbit monoclonal [EPR427 (N)] to mTOR (phospho S2481) Host species Rabbit Tested applications Suitable for: WB, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP Species reactivity Reacts with: Human sone backingWebmTOR is a member of the PI3 family of kinases and plays a critical role in regulating cell proliferation and metabolism in response to nutrient availability, cellular energy status, … soneath pondWebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. soneatly.comWebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor … soneath pond md ri