Thioltracker violet
WebApr 18, 2008 · Invitrogen Corporation announced the launch of ThiolTracker™ Violet, a thiol-reactive fluorescent probe that is useful for detecting intracellular reduced glutathione, … WebA collection of spectra representing the microscope Nikon Ni on FPbase. Note: When scaling to EC or QY, dyes/probes lacking EC/QY values in the database will disappear on the graph!
Thioltracker violet
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WebMar 10, 2024 · ThiolTracker™ Violet probe can stain the intracellular GSH, which was further utilized to detect the intracellular GSH after incubation with BSO or PEG 113-b-PBEMA 60 micelles. Apparently, the fluorescence intensity of A549R cells was much higher than that in A549 cells. WebMar 7, 2024 · MuSCs were immediately stained with ThiolTracker Violet for 20 minutes (5 μM, ThermoFisher) and resorted by FACS. For young MuSCs, all cells were re-collected (almost all are GSH high). For old MuSCs, the population was split into GSH high and GSH low collection groups. RNA-Seq libraries were constructed and sequenced with the …
WebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free … WebA collection of spectra representing the microscope Nikon Ti2 on FPbase.
WebNov 10, 2024 · After removal of the supernatant, cells were stained with ThiolTracker™ Violet for 20 min, following by the observation with fluorescence microscope and quantification with flow cytometry. 2.5.9 Investigation of intracellular reactive oxygen species level. CT26 cells pre-seeded in 24-well culture plate ... WebJan 8, 2024 · a Fluorescence micrographs of sympathetic neurons receiving the indicated treatments and then loaded with the GSH-sensitive dye, ThiolTracker™ Violet. b Quantification of the ThiolTracker™ Violet intensities in sympathetic neurons exposed to the indicated treatments. NGF withdrawal (20 h) slightly increased the basal GSH levels …
WebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free …
WebMar 2, 2024 · To stain the cells, 5 μl of suspensions were added to 100 μl plasma-like medium and pre-loaded with either 20 μM ThiolTracker Violet or 7.5 μM DHR or 5 μM DAF-FM DA for 30 min at 37°C in the darkness. Thereafter, RBC fluorescence was assessed. Data analyses were performed using Kaluza (Beckman Coulter) or FlowJo (Becton Dickinson ... pcn flow chartWebJul 9, 2024 · ThiolTracker Violet (# T10095, Thermo Fisher Sci.) is an intracellular thiol probe for the GSH detection. Since GSH represents the majority of intracellular free thiols in the DRGs, ThiolTracker Violet (1 µl) was used in estimating the intracellular level of GSH, following the manufacturer’s instruction. ... pcnf onlineWebThiolTracker Violet is a fluorescent compound with an excitation peak at 404 nm and an emission peak at 526 nm, giving it a fairly large Stokes' Shift of 122nm. It can be excited … scrub tech imagesWebThioltracker Violet, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … pcn flushingWebThiolTracker™ Violet 是一种更明亮和更稳定的细胞内硫醇探针，适用于可重现的 GSH 检测。 由于还原型谷胱甘肽代表细胞中的大多数细胞内游离硫醇，因此可利用 … scrub tech jobs little rock arkansasWebTo estimate the cellular level of reduced glutathione, HepG2 cells were seeded on poly-lysine coated 96 well plates at a density of 8000 cells/well and labeled with ThiolTracker™ Violet dye. In brief, once cells had been treated with 1 and 5 μM CC1 for 24 and 48 h the incubation medium was removed from the wells and cells were rinsed twice ... scrub tech memesWebOxidative stress is detected by measurement of reactive oxygen species (ROS) In normal/healthy cells, ROS is produced at a controlled rate Under conditions of oxidative stress, ROS production is dramatically increased, resulting in alteration of membrane lipids, proteins and nucleic acids Oxidative damage is associated with a number of pathological … scrub tech income