Websignal-to-ratio for THP-1 chemotaxis was achieved at approximately 25 minutes following exposure to the chemoattractant in the bottom chamber. Further incubation led to a … WebCCL5 Attracts THP-1-Derived Macrophages and Induces Their Polarity. Human THP-1 cells were added to the upper chamber of the transwell plate. After 4 h, the THP-1 cell count in the CCL5-treated group increased dramatically . In addition, The transwell assay was conducted by adding macrophages to the upper chamber.
CC chemokine receptor 7 promotes macrophage recruitment and …
WebJun 14, 2024 · Using a 0.4 µm Transwell coculture system (Sigma-Aldrich; Merck KGaA), THP-1 cells and TAMs were indirectly cocultured with A549 cells, respectively. A549 cells … WebJul 14, 2024 · Porphyromonas gingivalis (P. gingivalis) is one of the main periodontal bacteria. This pathogen was reported to enhance monocyte migration and adhesion to … seidy lopez age
Frontiers TILRR Promotes Migration of Immune Cells Through …
Webthe migration of another THP1 monocyte cell line that stably expresses GFP for visualization.41 The top chambers of the transwells were filled with equal numbers of a … WebWestern blot was performed to detect JMJD1A, JMJD1C, H3K9me2, and H3K9me3 expressions. A transwell migration assay was conducted to detect THP1-Mφ chemotaxis toward chemokine ligand 3 (CCL3). A chromatin immunoprecipitation (ChIP) assay was performed to detect H3K9me2-CCR5 complexes in THP1- Mφs. WebMar 3, 2024 · THP-1 cells were purchased from ATCC, cultured in RPMI 1640 with 10% FBS and supplemented with 0.05 mM β-mercaptoethanol (β-ME). To generate M0 cells, 1 × 10 6 THP-1 cells were suspended in medium with 150 ng/mL PMA (Sigma Aldrich, P8139) for 48 hours and then replaced with fresh medium without β-ME for 24 hours in 0.4 μm … seifer name meaning